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KMID : 0613820190290111218
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Journal of Life Science
2019 Volume.29 No. 11 p.1218 ~ p.1226
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Anti-inflammatory Activity of Antimicrobial Peptide Protaetiamycine 2 Derived from the Protaetia brevitarsis seulensis
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Lee Joon-Ha
Baek Min-Hee
Lee Hwa-Jeong
Kim In-Woo
Kim Sun-Young
Seo Min-Chul
Kim Mi-Ae
Kim Seong-Hyun
Hwang Jae-Sam
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Abstract
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The white-spotted flower chafer Protaetia brevitarsis seulensis is a medicinally beneficial and important edible insect species. We previously performed an in silico analysis of the Protaetia brevitarsis seulensis transcriptome to identify putative antimicrobial peptides and then tested their antimicrobial and hemolytic activities. These peptides had potent antimicrobial activities against bacteria and yeast without inducing hemolysis. In the present study, the cationic antimicrobial peptide, protaetiamycine 2, was selected for further assessment of its anti-inflammatory properties in mouse macrophage Raw264.7 cells. Protaetiamycine 2 treatment of Raw264.7 cells suppressed LPS-induced nitric oxide production and reduced the expression of inducible nitric oxide synthase and cyclooxygenase-2, as determined by real-time PCR and western blotting. The expression of proinflammatory cytokines (TNF-α, IL-6, and IL-1β) was also attenuated through the MAPKs and NF-κB signaling. We also confirmed that protaetiamycine 2 bound to bacterial cell membranes by a specific interaction with LPS. Collectively, these data obtained from LPS-induced Raw264.7 cells indicated that protaetiamycine 2 could have both antimicrobial and anti-inflammatory properties.
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KEYWORD
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Antimicrobial peptide, antimicrobial activity, anti-inflammatory activity, Protaetia brevitarsis seulensis, RNA sequencing
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